Composite

Part:BBa_J428100

Designed by: 2021_Engineering_Committee   Group: 2021 Engineering Committee   (2022-06-06)
Revision as of 17:55, 5 July 2022 by Vinoo (Talk | contribs)


Interlab_NegC

Non-fluorescent negative control for InterLab studies 2022. It uses the same elements as the fluorescent constructs but expresses a non-fluorescent protein (LuxR). It includes a region upstream of the promoter to insulate transcription, a promoter (J23106), a RiboJ to insulate translation, the B0032 RBS, the CDS for the LuxR protein, and an efficient transcriptional terminator (rnpB_T1). It imposes the same burden on the bacteria for a more fair growth comparison between the different devices.

Specially designed for the Interlab_2022_DoubleConstructs and Interlab_2022_ThreeColors studies.

It can be used as negative control for fluorescent measurements in general.

J428100.
Figure 1. SBOL Visual representation of the device J428100 from 2022 Interlab studies.


Interlab 2022 Experiment 1 Device

The following device is used in the iGEM 2022 InterLaboratory Study for Experiment 1.


Interlab 2022 Experiment 2 Device

The following device is used in the iGEM 2022 InterLaboratory Study for Experiment 2.


Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 71
    Illegal NheI site found at 94
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



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Categories
Parameters
None