![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Pre A2
Part:BBa_K4060101:Experience
Designed by: YU CHUAN LEE Group: iGEM21_NYCU-Taipei (2021-09-30)
Revision as of 14:02, 7 December 2021 by Clairechen (Talk | contribs)
Assembly of Pre A2 (pSB1C3)
The gene fragment Pre A2 is composed of pTetR BBa_R0040 and a double terminator BBa_B0015. We digested the vector fragment BBa_R0040 with XbaI and EcoRI and the insert fragment BBa_B0015 with EcoRI and SpeI. We ligated these fragments together to form Pre A2.
![](https://2021.igem.org/wiki/images/3/35/T--NYCU-Taipei--0A45820D-B05E-44B2-BD88-998A183211BB.png)
Applications of BBa_K4060101
User Reviews
UNIQabe7f2467f2f1df0-partinfo-00000003-QINU UNIQabe7f2467f2f1df0-partinfo-00000004-QINU