![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Pre A2
Part:BBa_K4060101:Experience
Designed by: YU CHUAN LEE Group: iGEM21_NYCU-Taipei (2021-09-30)
Assembly of Pre A2 (pSB1C3)
The gene fragment Pre A2 is composed of pTetR BBa_R0040 and a double terminator BBa_B0015. We digested the vector fragment BBa_R0040 with XbaI and EcoRI and the insert fragment BBa_B0015 with EcoRI and SpeI. We ligated these fragments together to form Pre A2.
![](https://2021.igem.org/wiki/images/3/35/T--NYCU-Taipei--0A45820D-B05E-44B2-BD88-998A183211BB.png)
Fig.3 Agarose electrophoresis in order to verify Pre A2. Lane 1, 100bp ladder. Lane 1’, partial of Life 1 kb Plus ladder. Lane 2,3, Pre A2 digested with EcoRI PstI.
Applications of BBa_K4060101
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