Coding

Part:BBa_K3861004

Designed by: Mario Delgadillo   Group: iGEM21_Humboldt_Berlin   (2021-10-01)
Revision as of 22:30, 21 October 2021 by KristinHU (Talk | contribs)

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lldR

lldR coding sequence from Salmonella Typhimurium ATCC14028. LldR regulates the transcription of the lldPRD operon which is involved in lactate metabolism. LldR represses the expression of the the lldRPD operon genes by binding to two operator regions of PlldR promoter and forming a loop.1,2. The lldR gene will be constituvely expressed in our designed backbone pKF01 (BBa_K3861005), and will allow for tight regulation of genes under the lldR promoter (BBa_K1847008).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Gillis, C. C. et al. Host-Derived Metabolites Modulate Transcription of Salmonella Genes Involved in l-Lactate Utilization during Gut Colonization. Infect. Immun. 87, e00773-18 (2019). 2. 1. Aguilera, L. et al. Dual role of LldR in regulation of the lldPRD operon, involved in L-lactate metabolism in Escherichia coli. J. Bacteriol. (2008) doi:10.1128/JB.02013-07.

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