Coding
Part:BBa_K3783003:Experience
Designed by: Satvik Kethireddy Group: iGEM21_OhioState (2021-10-21)
Revision as of 21:41, 21 October 2021 by Satvik k (Talk | contribs) (→Applications of BBa_K3783003)
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Applications of BBa_K3783003
OhioState 2021=
<figure>
<img src="" class="generalimage" alt="LAL Assay Graph"> <figcaption style="text-align:center">Figure 1. Endotoxin Units of Construct Lysates via LAL Assay</figcaption>
</figure>
The 2021 OhioState team characterized this biobrick using a Pierce Chromogenic Endotoxin Quantification Kit from ThermoFisher. The results were treated with Limulus Amebocyte Lysate which binds endotoxin and then attached to a chromogenic substrate which allowed us to quantify our results with provided standards. Cultures diluted to the -5 were used to quantify endotoxin concentrations. A control without any anti-lipid A proteins was also used as a comparison. There was a noticeable decrease in bound endotoxin.
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