Coding

Part:BBa_K3783003:Experience

Designed by: Satvik Kethireddy   Group: iGEM21_OhioState   (2021-10-21)


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OhioState Application

LAL Assay Graph
Figure 2. Endotoxin Units of Construct Lysates via LAL Assay

The 2021 OhioState team characterized this biobrick using a Pierce Chromogenic Endotoxin Quantification Kit from ThermoFisher. The results were treated with Limulus Amebocyte Lysate which binds endotoxin and then attached to a chromogenic substrate which allowed us to quantify our results with provided standards. Cultures diluted to the -5 were used to quantify endotoxin concentrations. A control without any anti-lipid A proteins was also used as a comparison. There was a noticeable decrease in bound endotoxin.



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