Part:BBa_K592015

amilCP Blue Coding Device

This color expressing cassette is a potential alternative/complementary to BBa_J04450, the current RFP cassette in all of the BioBrick backbones. Bacterial colonies exhibit strong blue color after 18 hours. Due to the much darker blue/purple color tone, even partially blue colonies are readily recognized.

We found this design very useful when assembling components that contain RFP, mCherry, etc. The assembled parts are not recognizable from the background insert if all colonies becomes red after transformation. Luckily, very few genes produce substances that make the cells turn blue/purple, which makes our construct useful. Due to the strong promoter, over-expressing amilCP will be costly to the cells.

The promoter is constitutive, the RBS is strong, the double terminator is not BBa_B0015 but BBa_B0014.

The images above show E coli constitutively expressing amilCP BBa_K592009 (blue), amilGFP BBa_K592010 (yellow), and RFP BBa_E1010 (red).

Improvement by ICJFLS2021

Overview:

BBa_K592015 is a amilCP blue coding device, which consists of J23101, B0034, K592009, and B0014. It was constructed by iGEM11_Uppsala-Sweden in Sep. 2011. This device can express blue/purple color protein, which enriches the reporter genes for the research of life science. K592009 is a blue chromo protein gene from the Acropora millepora. AmilCP, naturally exhibits strong color when expressed. The protein has an absorbance maximum at 588 nm giving it a blue/purple color visible to the naked eye, thereby requiring no instruments to observe. The strong color is readily observed in both LB or agar culture, in less than 24 hours of incubation. It is a very useful part.