Part:BBa_K3733042
PhoA-azurin with a His-tag
Azurin is a copper-containing redox protein secreted by Pseudomonas aeruginosa. Azurin preferentially enters a variety of cancer cells and induces apoptosis or cell cycle arrest, thereby playing an anti-cancer effect. A 6×His tag was added in order to purify the protein. PhoA, a signal peptide of Sec pathway in Escherichia coli, have the capability to guide the LL-37 excrete out of the cell.
To further demonstrate that Azurin generated from our engineered bacteria could combine with p53 successfully, we mixed Azurin(without 6x His tag) with p53(with 6x His tag) and purified them together by a Ni-NTA column. The SDS-PAGE gel of combination verification shows below in Figure 16. Two correct stripes can be observed in the lane of the gel, which illustrates these two proteins can combine with each other.
Sequence and Features
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Reference
Huang F, Qianhui S, Zhaojie Q, et al. Anticancer actions of azurin and its derived peptide p28[J]. The protein journal, 2020, 39(2): 182-189.
| None |