Part:BBa_K3733042
PhoA-azurin with a His-tag
Azurin is a copper-containing redox protein secreted by Pseudomonas aeruginosa. Azurin preferentially enters a variety of cancer cells and induces apoptosis or cell cycle arrest, thereby playing an anti-cancer effect. A 6×His tag was added in order to purify the protein. PhoA, a signal peptide of Sec pathway in Escherichia coli, have the capability to guide the LL-37 excrete out of the cell.
Functional Parameters
To demonstrate that our engineered bacteria could systhesis Azurin successfully, we designed this composite part and transformed the plasmid into E.coli BL21. The phoA label is utilized to assist Azurin being secreted into the medium, and His tag could combine with a Ni-NTA column. We purified Azurin by a Ni-NTA column and the SDS-PAGE gel is shown below in Figure 1. Correct stripe can be observed in the lane of the gel, which illustrates we obtained Azurin successfully.
Sequence and Features
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Reference
Huang F, Qianhui S, Zhaojie Q, et al. Anticancer actions of azurin and its derived peptide p28[J]. The protein journal, 2020, 39(2): 182-189.
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