Coding

Part:BBa_K3861004

Designed by: Mario Delgadillo   Group: iGEM21_Humboldt_Berlin   (2021-10-01)
Revision as of 15:52, 21 October 2021 by Martje1998 (Talk | contribs)


lldR

lldR coding sequence from Salmonella typhimurium ATCC14028. LldR regulates the transcription of the LldPRD operon which is involved in lactate metabolism. LldR is represses the expression of the the lldRPD operon genes by binding two operator regions and forming a loop.1,2. The lldR gene will be constituvely expressed in our designed backbone pKFO1 (BBa_K3861005), and will allow for tight regulation of genes under the lldR promoter (BBa_K1847008).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Gillis, C. C. et al. Host-Derived Metabolites Modulate Transcription of Salmonella Genes Involved in l-Lactate Utilization during Gut Colonization. Infect. Immun. 87, e00773-18 (2019). 2. 1. Aguilera, L. et al. Dual role of LldR in regulation of the lldPRD operon, involved in L-lactate metabolism in Escherichia coli. J. Bacteriol. (2008) doi:10.1128/JB.02013-07.

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