Composite

Part:BBa_K4015015

Designed by: Andrew Peng Yi   Group: iGEM21_KEYSTONE   (2021-10-21)
Revision as of 14:41, 21 October 2021 by Anrdew (Talk | contribs)


Lcp-HlyA

Lcp-HlyA is composed of two basic parts, Lcp1VH2 and HlyA. HlyA and Lcp are connected by Gibson assembly in order to allow Lcp to be secreted outside the cell through HlyA. HlyA's C-terminal is fused with Lcp1VH2.


Usage and Biology

From the literature, we know that Lcp1VH2 possesses water solubility (andler, et al, 2019). Also, our experiments tell us that lcp is extremely difficult to purify. Therefore, we tried to use signal peptide and secretion system to cooperate to secrete the effective protein out of the cell.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 508
    Illegal XhoI site found at 2033
    Illegal XhoI site found at 2187
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 127
    Illegal NgoMIV site found at 485
    Illegal NgoMIV site found at 1679
    Illegal NgoMIV site found at 2001
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 112
    Illegal BsaI.rc site found at 1582
    Illegal SapI.rc site found at 100


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Parameters
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