Composite
Part:BBa_K4015015
Designed by: Andrew Peng Yi Group: iGEM21_KEYSTONE (2021-10-21)
Lcp1VH2-HlyA
Lcp1VH2-HlyA is composed of two basic parts, Lcp1VH2 and HlyA. HlyA and Lcp are connected by Gibson assembly in order to allow Lcp to be secreted outside the cell through HlyA. HlyA's C-terminal is fused with Lcp1VH2.
Usage and Biology
From the literature, we know that Lcp1VH2 possesses water solubility (andler, et al, 2019). Also, our experiments tell us that lcp is extremely difficult to purify. Therefore, we tried to use signal peptide and secretion system to cooperate to secrete the effective protein out of the cell.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 508
Illegal XhoI site found at 2033
Illegal XhoI site found at 2187 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 127
Illegal NgoMIV site found at 485
Illegal NgoMIV site found at 1679
Illegal NgoMIV site found at 2001 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 112
Illegal BsaI.rc site found at 1582
Illegal SapI.rc site found at 100
[edit]
Categories
Parameters
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