Composite

Part:BBa_K4011013

Designed by: Xiaochong Ma   Group: iGEM21_LINKS_China   (2021-10-21)
Revision as of 11:36, 21 October 2021 by Registry (Talk | contribs)

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ptac-RiboJ-TnaA-RL-FMO-B0015

pTac-RiboJ-TnaA-RL-FMO-B0015 is an expression cassette in E. coli expressing TnaA-RL-FMO No part name specified with partinfo tag. used to convert 6-Halogen-Trpytophan (6-X-Trp) into di-halogenated indigoid dyes such as tyrian purple. TnaA-RL-FMO is composed of two seperate domains: TnaA and FMO, fused with a flexible linker. pTac-RiboJ BBa_K3552015 is an IPTG-inducible promoter suited to all strains of No part name specified with partinfo tag.. B0015 BBa_B0015 is a strong terminator. This is a part in a part collection where we enable the production of indigo, tyrian purple, and related dyes from tryptophan in E. coli.

The part collection includes: Parts expressing Fre-SttH to convert Trp to 6-X-Trp. BBa_K4011003 and BBa_K4011012. Parts expressing fusion protein TnaA-FMO to convert 6-X-Trp into indigoid dyes. BBa_K4011004, BBa_K4011005, BBa_K4011013, BBa_K4011014, BBa_K4011015, and BBa_K4011019.

Our part collection can be used to help and inspire future teams to design and perfect different indigoid dye production pathways in E. coli, adding to the collection.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4158
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2565
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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