Coding

Part:BBa_K3765010

Designed by: Dipayan Akhuli   Group: iGEM21_IISc-Bangalore   (2021-10-19)
Revision as of 06:57, 21 October 2021 by Dipayan akhuli (Talk | contribs)

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sfGFP (Superfolder GFP)

sfGFP (Superfolder GFP) is a green fluorescent protein, which unlike the normal GFP, can retain its fluorescence even when fused with poorly folding proteins, because of its robust folding. So it is often used as a fluorescent tag for large proteins. This part is an E. coli codon-optimized version of BBa_I746916 minus the last two amino acids.

Usage and Biology

This part was included in our OpdA-SpyTag002 construct for easy detection of the fusion protein and also for assaying the binding between this fusion protein and the SpyCatcher002-dCBD fusion protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 627
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/reporter/fluorescence
Parameters
None