Composite

Part:BBa_K4032101

Designed by: Tsugumi Ohzeki   Group: iGEM21_Gunma   (2021-09-30)
Revision as of 11:27, 20 October 2021 by Tsubasa O (Talk | contribs)


lacI+RBS+α-gal-RFP+double terminator

Contents :

・The lac promoter (from BBa_R0010)

・The RBS (from BBa_B0034)

・The gene of the α-galactosidase-RFP fusion protein.

・The double terminator (from BBa_B0015)


Usage and Biology

This enzyme catalyzes the hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.

Red fluorescence of RFP is observed under the fluorescence microscope and natural light.


Design

This part expresses the α-galactosidase-RFP fusion protein.

α-galactosidase is from E.coli K-12 MG1655 and RFP is from No part name specified with partinfo tag.


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