Translational_Unit
RBS amyRFP

Part:BBa_K4032006

Designed by: Tsugumi Ohzeki   Group: iGEM21_Gunma   (2021-09-30)
Revision as of 07:57, 20 October 2021 by Tsugumi (Talk | contribs)


RBS+amylase-RFP

NC_000913.3 malS alpha-amylase [Escherichia coli str. K-12 substr. MG1655] - Gene - NCBI (nih.gov)

Contents :

・The gene codes for the amylase-RFP fusion protein.

・The native RBS(Part:BBa K523001 - parts.igem.org)


Usage and Biology 

      This enzyme hydrolyzes of (1-4)-alpha-D-glycosidic linkages in polysaccharides containing three or more (1-4)-alpha-linked D-glucose units. See details NC_000913.3 (https://www.ncbi.nlm.nih.gov/gene/948088).

      In the case of this part, Red red fluorescence of RFP is observed under the fluorescence microscope, but isn’t observed under natural light.


The lac promoter and double terminator are added to this part forming BBa_K4032xx .


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1233
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 369
    Illegal AgeI site found at 1534
    Illegal AgeI site found at 2613
    Illegal AgeI site found at 2725
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None