Translational_Unit
RBS amyRFP

Part:BBa_K4032006

Designed by: Tsugumi Ohzeki   Group: iGEM21_Gunma   (2021-09-30)


RBS+amylase-RFP

Contents :

・The gene cording for the amylase-RFP fusion protein (BBa_K4032003).

・The native RBS from BBa_K523001

For more information on the amylase gene malS, see NCBI: NC_000913.3

Usage and Biology

This enzyme hydrolyzes of (1-4)-α-D-glycosidic linkages in polysaccharides containing three or more (1-4)-α-linked D-glucose units. For more information, See NCBI: NC_000913.3.

In the case of this part, Red red fluorescence of RFP is observed with a fluorescence microscope, but isn’t observed under the natural light source.


The lac promoter and double terminator are added to this part, forming BBa_K4032104 .


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1233
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 369
    Illegal AgeI site found at 1534
    Illegal AgeI site found at 2613
    Illegal AgeI site found at 2725
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None