Composite

Part:BBa_K3889100

Designed by: Prameya Garge, Ashwin Sharma   Group: iGEM21_IISER-Tirupati_India   (2021-10-08)
Revision as of 11:06, 19 October 2021 by AshWinShaRma (Talk | contribs)


SRTF1 expression cassette Bacillus subtilis

SRTF1 is expressed under a constitutive promoter SP126 (BBa_K3889010). In absence of progesterone it can bind to its binding site (BBa_K3889030) and repress transcription of the gene.Now as progesterone enters the system SRTF1 binds to progesterone leaving its binding site free for the transcription machinery to start working. This results in a progesterone inducible gene expression.

This part along with BBa_K3889101 and BBa_K3889102 was used in order to produce Ovastacin (BBa_K3889022) when progesterone in absent in the fallopian tube. The working of the system is shown below: Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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