Coding

Part:BBa_K3952001:Design

Designed by: B. Liam, D. Amanda, R. Jazmine, S. Brianna   Group: iGEM21_SUNY_Oneonta   (2021-10-01)
Revision as of 15:44, 18 October 2021 by Jazmine124 (Talk | contribs)


APOB-100 (Cholesterol Deficiency Allele)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 330
    Illegal XhoI site found at 591
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 853


Design Notes

This is the negative control of the APOB gene that will be used within a Flappase genetic testing system.


A SpaI restriction site was found at 853. To adhere to RCF1000 assembly, a silent mutation was applied to the codon located within 855 and 857. The original codon was AGA and was altered to AGG.


Source

The source of the APOB-100 sequence is the genomic sequence from Bos taurus, apolipoprotein B genome

References

Through the assistance provide by Team Rochester, we were able to acquire the ERV2-1 sequence from their subscription to Repbase. Repbase is a database consisting of repetitive DNA elements.