Coding

Part:BBa_K3952001:Design

Designed by: B. Liam, D. Amanda, R. Jazmine, S. Brianna   Group: iGEM21_SUNY_Oneonta   (2021-10-01)


APOB-100 (Cholesterol Deficiency Allele)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 330
    Illegal XhoI site found at 591
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 853


Design Notes

This is the positive control of the APOB gene that will be used within a Flappase genetic testing system.


An illegal SpeI restriction site was found to be present in the ERV2-1 transposon insert. To alter this to make the part compatible with the RCF10 assembly, the sequence ACTAGT within the insert has been changed to GCTAGT. This eliminates the illegal restriction site.

Source

The source of the APOB-100 sequence is the genomic sequence from Bos taurus, apolipoprotein B genome.

References

Through the assistance provided by Team Rochester, we were able to acquire the ERV2-1 sequence from their subscription to Repbase. Repbase is a database consisting of repetitive DNA elements.