Part:BBa_K3889085

P22 binding site F

P22 c2 repressor DNA binding site.

Part Name	Part number	Sequence	Rel KD	KD (in M)
P22 binding site A	BBa_K3889080	ATTTAAGATATCTTAAAT	1	1.6 × 10-8
P22 binding site B	BBa_K3889081	AATTAAGATATCTTAATT	1.8	2.88 × 10-8
P22 binding site C	BBa_K3889082	ATTTAAGAATTCTTAAAT	2	3.2 × 10-8
P22 binding site D	BBa_K3889083	AGTTAAGATATCTTAACT	2.6	4.16 × 10-8
P22 binding site E	BBa_K3889084	ATTAAAGATATCTTTAAT	3.8	6.08 × 10-8
P22 binding site F	BBa_K3889085	ACTTAAGATATCTTAAGT	4.3	6.88 × 10-8
P22 binding site G	BBa_K3889086	ATTCAAGATATCTTGAAT	5	8.0 × 10-8
P22 binding site H	BBa_K3889087	ATTGAAGATATCTTCAAT	7.6	1.216 × 10-7
P22 binding site I	BBa_K3889088	ATTTAAGAGCTCTTAAAT	10	1.6 × 10-7
P22 binding site J	BBa_K3889089	ATTTAAGACGTCTTAAAT	10	1.6 × 10-7
P22 binding site K	BBa_K3889090	ATTTACGATATCGTAAAT	30	4.8 × 10-7
P22 binding site L	BBa_K3889091	ATTTAAAATATTTTAAAT	55	8.8 × 10-7

Fig.1:Kd values of P22 binding site

Usage and Biology

Different binding affinities of a repressor provides a variable system that can be used for different types of systems. As K_D increases the dissociation of repressor from the DNA sequence also increases providing with lower repression. P22 repressor (BBa_K3889020) binds to this sequence as a dimer. This inhibits the enzymes from transcription the genes on whose promoter this operator site is present. Hence this could be fused with any promoter in order to form a repressible system as done in BBa_K3889102.

Graph

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

1. Watkins, D., Hsiao, C., Woods, K. K., Koudelka, G. B., & Williams, L. D. (2008). P22 c2 Repressor−Operator Complex:  Mechanisms of Direct and Indirect Readout. Biochemistry, 47(8), 2325–2338. https://doi.org/10.1021/bi701826f