Part:BBa_K3889012

Bacillus subtilis Synthetic Promoter 200

This is a synthetically derived promoter sequence for the expression of genes in Bacillus subtilis.

Promoter	Sequence 5‘ → 3‘	Relative activity wrt P43 of GFP (%)	Standard deviation
SP126	AAAAATTATAAAAATGTGTTGACAAAGGGGGTCCTGTATGTTATAATAGCTT	29.07	0.23
SP146	AAAAATAACAAAAACGTGTTGACAATAAAGATTAACCGTGATATAATTAAAT	40.39	0.69
SP200	AAAAATTAGAAAAATGTGTTGACACTCGGACGAAACAATGGTATAATGGCAA	76.82	0.9

Synthetic Promoters data from SP1-214[1]

Usage and Biology

SP126(BBa_K3889010), SP146(BBa_K3889011), SP200(BBa_K3889010) are a part of synthetic library promoters from Liu et al. (2018)[1]. These synthetic promoters are characterised to have constitutive expression, hence they can be used in general protein production. Since the library contains 214 synthetic promoters providing variability and robustness in expression, according to the system's need. All SP promoters have the following consensus sequences:

Based on this the promoters are as follows:

Modelling

We planned to use SP200 with BBa_K1351031 for gene expression of mf-Lon (BBa_K3889101).

Parameters used:

Rate of P22 production = 9218880/nM*day

Rate of P22 degradation = 1.69/day

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

1. Liu, D., Mao, Z., Guo, J., Wei, L., Ma, H., Tang, Y., Chen, T., Wang, Z., & Zhao, X. (2018). Construction, Model-Based Analysis, and Characterization of a Promoter Library for Fine-Tuned Gene Expression in Bacillus subtilis. ACS Synthetic Biology, 7(7), 1785–1797. https://doi.org/10.1021/acssynbio.8b00115