Part:BBa_K3889011

Bacillus subtilis Synthetic Promoter 146

This is a synthetically derived promoter sequence for constitutive expression of genes in Bacillus subtilis.

Promoter	Sequence 5‘ → 3‘	Relative activity wrt P43 of GFP (%)	Standard deviation
SP126	AAAAATTATAAAAATGTGTTGACAAAGGGGGTCCTGTATGTTATAATAGCTT	29.07	0.23
SP146	AAAAATAACAAAAACGTGTTGACAATAAAGATTAACCGTGATATAATTAAAT	40.39	0.69
SP200	AAAAATTAGAAAAATGTGTTGACACTCGGACGAAACAATGGTATAATGGCAA	76.82	0.9

Synthetic Promoters data from SP1-214[1]

Usage and Biology

SP126(BBa_K3889010), SP146(BBa_K3889011) and SP200(BBa_K3889012) are a part of synthetic library promoters from Liu et al. (2018)[1]. These synthetic promoters are characterised to have constitutive expression, hence they can be used in general protein production. The library contains 214 synthetic promoters providing variability and robustness in expression, according to the system's need. All SP promoters have the following consensus sequences:

Based on this the promoters are as follows:
Fig source: Geneious version 2021.2 created by Biomatters. Available from https://www.geneious.com

Modelling

We planned to use SP146 with BBa_K1351030 for gene expression of mf-Lon (BBa_K3889122).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

1. Liu, D., Mao, Z., Guo, J., Wei, L., Ma, H., Tang, Y., Chen, T., Wang, Z., & Zhao, X. (2018). Construction, Model-Based Analysis, and Characterization of a Promoter Library for Fine-Tuned Gene Expression in Bacillus subtilis. ACS Synthetic Biology, 7(7), 1785–1797. https://doi.org/10.1021/acssynbio.8b00115