Part:BBa_K3893027

AHL induced expression of Lysis protein.

Figure 1. SBOL Visual representation of the part

AHL induced transcriptional unit assembled with a one-pot Level 1 Golden Gate reaction using BsaI type IIS endonuclease.

This composite part, together with K3893026, is a component of the intermediate part [1] to assemble the release system device ([2]) of our Agrobactory 593. It is used to express the lysis protein Phi174 when AHL (bound to to LuxR) is present, a fundamental piece of our population oscillator. release device.

This transcriptional unit is composed of the following standardized parts from Valencia_UPV 2018 iGEM Team [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:

• BBa_K2656003: the R0062 inducible pLux promoter in its Golden Braid compatible version
• BBa_K2656010: the B0032 weak ribosome biding site in its Golden Braid compatible version
• BBa_K2656015: the Pho174 lysis protein
• Terminator BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version

Part BBa_K2656015 is the lysis gene from the enterobacteria phage phiX174 coding sequence. The lysis gene E of coliphage PhiX174 encodes a 91-amino acid (aa) residue protein of approximately 10 kDa that mediates inhibition of peptidoglycan biosynthesis of Gram-negative bacteria membrane.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]