Composite
Part:BBa_K3895009
Designed by: Rui Zhu Group: iGEM21_SZ_SHD (2021-10-01)
Revision as of 13:47, 15 October 2021 by Tigerjiang (Talk | contribs)
kerBlMKU3_dna_pET-28a(+)
ggggg
Construction
Keratinase KerBlMKU3 (BBa_K3895005) is a serine keratinase originally secreted by Bacillus licheniformis MKU3. It can work at 37°C for 48 h in nutrient broth or on feather medium [1]. The keratinase was compared with the other 3 keratinases (BBa_K3895004; BBa_K3895005;BBa_K3895006) with the same construction method. 6x His-tags were added to both sides of the sequence for purification and constructed into PET-28a(+). The constitutive T7 promoter (BBa_I719005) derived from the T7 bacteriophage allows high expression of proteins.
Name of keratinase | IPTG concentration/mM | Temperature/°C | Time/h |
---|---|---|---|
KerBIMKU3 | 0.4 | 37 | 4 |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 606
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 411
Illegal AgeI site found at 1149 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 351
[edit]
Categories
Parameters
//awards/composite_part/nominee
None |