Composite

Part:BBa_K4040023

Designed by: Tong Yin   Group: iGEM21_NMU_China   (2021-09-27)
Revision as of 12:38, 11 October 2021 by Ytong (Talk | contribs)


pSV40-UAS-GFP Expression Cassette

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1115

Usage and Biology

The composite part consists of seven parts: the promoter UAS-PSV40 (BBa_K4040033), GFP Genera (BBa_E0840), which initiates the expression of coding sequence. pCMV(BBa_I712004) was used as the promoter. The indicator which detects the expression of the pathway and the expression component of CAR-FcRγ: CD8a signal peptide (BBa_K4040014), CR3022 scFv (BBa_K4040019), CD8 Hinge (BBa_K4040004), CD8TM (BBa_K4040007), FcRγ (BBa_K4040002).  When the GAL4-KRAb fragment from IL-6 complex receptor BBa_K4040021 is released, it binds to the promoter UAS-PSV40 to inhibit the expression of CAR- FcRγ and reduce the transformation of macrophages into M1 state to alleviate the inflammatory response.

Figure 1.The coding of the CAR-gamma.
Figure 2.The structure and downstream pathway of the composite IL6R.



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