RBS

Part:BBa_K3740008

Designed by: Guiyi Huang   Group: iGEM21_SZPT-CHINA   (2021-08-26)
Revision as of 06:28, 10 October 2021 by HUANG (Talk | contribs)


RBS009

Description

Modulation of protein expression level

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


2021 SZPT-China

Biology

Mutagenesis at specific position of RBS (BBa_B0034) was achieved RBS009 (BBa_K3740008) using random primers and PCR, and the constructed plasmid J23100-RBS009-sfGFP-rrn B T1 (BBa_K3740060）. Compare the fluorescence intensity of sfGFP induced by J23100-B0034-sfGFP-rrn B T1 (BBa_K3740058)


Usage

[edit]
Categories
Parameters
None