Primer
pTF_tal_re

Part:BBa_K4047004:Design

Designed by: An Nguyen, Avery Imes   Group: iGEM21_MiamiU_OH   (2021-09-07)
Revision as of 18:29, 4 October 2021 by Anniequyan (Talk | contribs) (→‎Source)


primerTF_tal-reverse


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Generated from a combination of factors. Overlap sequence sourced from pAM2991. This sequence lies downstream of the trc promoter and is thus likely to contain a shine-dalgarno binding sequence, increasing the expression of fbp in the polycistronic mRNA produced. https://www.addgene.org/40248/

Annealing sequence sourced from Synechococcus elongatus PCC 7942 Genbank genome sequence for transaldolase, at the locus tag Synpcc7942_2297. https://www.ncbi.nlm.nih.gov/nuccore/CP000100.1?from=2367528&to=2368721&report=fasta&strand=2

References