Primer
pT_tal-rev

Part:BBa_K4047001

Designed by: An Nguyen, Avery Imes   Group: iGEM21_MiamiU_OH   (2021-09-03)
Revision as of 18:00, 4 October 2021 by Anniequyan (Talk | contribs)


primerT_tal-reverse


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 14
    Illegal PstI site found at 2
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 20
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 14
    Illegal PstI site found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 14
    Illegal PstI site found at 2
  • 1000
    COMPATIBLE WITH RFC[1000]


This is the reverse primer to amplify the gene for transaldolase. The overlap sequence (red) allows proper Gibson assembly with the pGEM-tal backbone. The annealing portion (all caps) allows annealing to the WT S. elongatus PCC 7942 genome for proper amplification.

[edit]
Categories
Parameters
None