Primer
pT_tal-rev
Part:BBa_K4047001
Designed by: An Nguyen, Avery Imes Group: iGEM21_MiamiU_OH (2021-09-03)
Revision as of 17:53, 4 October 2021 by Anniequyan (Talk | contribs)
This is the reverse primer to amplify the gene for transaldolase. The overlap sequence (red) allows proper Gibson assembly with the pGEM-tal backbone. The annealing portion (all caps) allows annealing to the WT S. elongatus PCC 7942 genome for proper amplification.
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Parameters
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