Primer
pT_tal-fwd

Part:BBa_K4047000

Designed by: Hope Kirby, Avery Imes   Group: iGEM21_MiamiU_OH   (2021-09-01)
Revision as of 17:45, 4 October 2021 by Anniequyan (Talk | contribs) (adding info)

This is the forward primer for transaldolase. The overlap sequence (acacaggaaacagaccatgg) allows proper Gibson assembly with the pGEM-tal backbone. The intermediate aattc sequence preserves the EcoRI restriction digest site for confirmation testing. The annealing portion (ATGGCAGCGAATTTACTCGAAC) allows annealing to the WT S. elongatus PCC 7942 genome for proper

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Categories
//primer/part
Parameters
None