Coding

Part:BBa_K3724010

Designed by: Tracey Moyston   Group: iGEM21_Rochester   (2021-09-30)
Revision as of 02:12, 1 October 2021 by Registry (Talk | contribs)

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Riboflavin biosynthesis protein RibF

Two extracellular electron transfer pathways have been identified in the reduction of graphene oxide (GO) by Shewanella oneidensis. These are indirect electron transfer, mediated by secreted electron shuttles, and direct extracellular electron transfer (DET) which involves direct contact with the extracellular material. It has been proposed that flavins may act as electron shuttles in the reduction of extracellular material by Shewanella oneidensis. These flavins can exit the cell as Flavin adenine dinucleotide (FAD) where they are then reconverted to riboflavin. RibF converts riboflavin to FAD to be shuttled out. Increasing expression of RibF should increase the conversion of riboflavin to FAD and would therefore increase the electron donors available for reduction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7
    Illegal BsaI.rc site found at 955


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Categories
Parameters
None