Plasmid

Part:BBa_K3779004:Design

Designed by: JiaCheng, Li   Group: iGEM21_NWU-CHINA-B   (2021-06-19)
Revision as of 17:52, 27 September 2021 by JunfengHui (Talk | contribs) (→‎Design Notes)

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pPICZαA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1257
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
    Illegal BamHI site found at 1677
    Illegal XhoI site found at 1184
    Illegal XhoI site found at 1246
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1224
    Illegal NgoMIV site found at 2435
    Illegal NgoMIV site found at 2496
    Illegal AgeI site found at 1396
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1660
    Illegal BsaI.rc site found at 1859
    Illegal BsaI.rc site found at 2857


Design Notes

gaattcgcggccgcttctagtactagaggaacaaaaactcatctcagaagaggatctgtactagagcatcatcatcatcatcattactagaggtttgtagccttagacatgactgttcctcagttcaagttgggcacttacgagaagaccggtcttgctagattctaatcaagaggatgtcagaatgccatttgcctgagagatgcaggcttcatttttgatacttttttatttgtaacctatatagtataggattttttttgtcattttgtttcttctcgtacgagcttgctcctgatcagcctatctcgcagctgatgaatatcttgtggtaggggtttgggaaaatcattcgagtttgatgtttttcttggtatttcccactcctcttcagagtacagaagattaagtgagaccttcgtttgtgcggatcctactagagcccacacaccatagcttcaaaatgtttctactccttttttactcttccagattttctcggactccgcgcatcgccgtaccacttcaaaacacccaagcacagcatactaaattttccctctttcttcctctagggtgtcgttaattacccgtactaaaggtttggaaaagaaaaaagagaccgcctcgtttctttttcttcgtcgaaaaaggcaataaaaatttttatcacgtttctttttcttgaaatttttttttttagtttttttctctttcagtgacctccattgatatttaagttaataaacggtcttcaatttctcaagtttcagtttcatttttcttgttctattacaactttttttacttcttgttcattagaaagaaagcatagcaatctaatctaaggggctactagaggtgttgacaattaatcatcggcatagtatatcggcatagtataatacgacaaggtgaggaactaaacctactagatggccaagttgaccagtgccgttccggtgctcaccgcgcgcgacgtcgccggagcggtcgagttctggaccgaccggctcgggttctcccgggacttcgtggaggacgacttcgccggtgtggtccgggacgacgtgaccctgttcatcagcgcggtccaggaccaggtggtgccggacaacaccctggcctgggtgtgggtgcgcggcctggacgagctgtacgccgagtggtcggaggtcgtgtccacgaacttccgggacgcctccgggccggccatgaccgagatcggggagcagccgtgggggcgggagttcgccctgcgcgacccggccggcaactgcgtgcacttcgttgccgaggagcaggactgatactagagcacgtccgacggcggcccacgggtcccaggcctcggagatccgtcccccttttcctttgtcgatatcatgtaattagttatgtcacgcttacattcacgccctccccccacatccgctctaaccgaaaaggaaggagttagacaacctgaagtctaggtccctatttatttttttatagttatgttagtattaagaacgttatttatatttcaaatttttcttttttttctgtacagacgcgtgtacgcatgtaacattatactgaaaaccttgcttgagaaggttttgggacgctcgaaggctttaatttgcaagcttactagatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcaatgctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacgggtactagaggactggttccaattgacaagctactagatgagatttccttcaatttttactgctgttttattcgcagcatcctccgcattagctgctccagtcaacactacaacagaagatgaaacggcacaaattccggctgaagctgtcatcggttactcagatttagaaggggatttcgatgttgctgttttgccattttccaacagcacaaataacgggttattgtttataaatactactattgccagcattgctaaagaagaaggggtatctctcgagaagagagaggctgaacgatactagagtattcttttccaaattcttttagattcggttggtctcaagctggttttcaatctgagatgggtactccaggatctgaagatccaaatactgattggtataagtgggttcatgacccagagaacatggctgctggtttggtttctggtgacttaccagaaaacggtcctggttactggggtaactacaagacttttcatgataacgctcaaaagatgggtttgaaaatcgctagattgaacgtcgaatggtctagaatctttcctaatccactaccaagacctcaaaattttgatgagtctaaacaagatgttactgaagttgaaattaatgagaatgaattgaaaagacttgatgaatacgctaacaaggatgcattgaaccactatagagaaatttttaaggatttgaagtctagaggattgtattttattttgaatatgtaccattggccattgcctttgtggttgcacgatccaattagagttagaagaggtgactttactggtccatctggatggctttctactagaaccgtttacgagtttgctagattttcagcttacattgcttggaaatttgatgatttggttgatgaatactctactatgaacgaacctaatgttgttggtggtttaggttatgttggagttaaatctggttttcctccaggatatttgtccttcgaattgtctagaagagctatgtataatattattcaagcacatgctagagcttatgacggtattaagtctgtttcaaagaaacctgttggaattatctacgctaattcatcttttcaacctttgaccgataaagacatggaagctgttgaaatggctgaaaacgataatagatggtggtttttcgatgctattattagaggtgagattaccagaggtaatgaaaagattgttagagatgacttgaagggtaggttggactggatcggtgttaattactacactagaactgttgttaagagaactgaaaagggttacgtgtcattaggaggttatggtcatggttgtgaaaggaactctgtttctttggctggtctgccaacttctgattttggttgggaattctttccagagggtttgtacgatgtgttgactaaatattggaacaggtatcatttgtacatgtatgttactgaaaacggtattgctgacgatgctgattaccaacgtccatactaccttgtttctcacgtttatcaagttcatagagctattaattcaggtgctgatgttagaggttacttgcattggtcattggctgataactacgaatgggcttctggattttctatgagatttggtttgttgaaggttgattataacactaaaagattatattggagacctagtgctttagtttacagagaaattgctactaacggagctataactgatgaaattgaacatttgaattcagttccaccagttaagccattgagacattaatactagtagcggccgctgcag

Source

Commercial synthetic

References