Regulatory

Part:BBa_K3866001

Designed by: Pericles Vasileiou   Group: iGEM21_Thessaly   (2021-09-21)
Revision as of 18:25, 25 September 2021 by Periclesv (Talk | contribs)


pTrc:RBS GB compatible with A1-B2

The trc promoter is a hybrid of the lac and trp promoters which is a stronger promoter in comparison to the lac promoter.

Usage and Biology

The trc promoter was created by insertion of 1 bp into the 16 bp sequence between the consensus -35 and -10 sequences of the tac promoter, to obtain the optimal consensus distance of 17 bp between the -35 and -10 signal. This promoter includes the Lac Operator sequence lacO, which can be bound by the Lac repressor lacI, as well as an RBS BBa_B0034

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None