Intermediate
KSin3

Part:BBa_K165028

Designed by: John Szymanski   Group: iGEM08_BrownTwo   (2008-10-27)
Revision as of 19:11, 2 November 2008 by Jszymans (Talk | contribs)

Kozak + Sin3 Repression Domain

This is the kozak translation initiation sequence ligated to the Sin3 Repression domain. It can be used to build synthetic transcriptional repressing factors by ligating it to any DNA-binding domain, optionally a tandem set of fluorescent proteins to track expression, and to the SV40 NLS +ADH1 terminator

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1775
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 946
    Illegal SapI.rc site found at 1539


[edit]
Categories
Parameters
None