Composite

Part:BBa_K3425060:Design

Designed by: Tereza Hubackova   Group: iGEM20_UofUppsala   (2020-10-25)
Revision as of 03:19, 28 October 2020 by Tereza H (Talk | contribs)


T7 polymerase regulated Juniper GFP substrate for Qβ replicase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 550
    Illegal XbaI site found at 83
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 550
    Illegal NheI site found at 859
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 550
    Illegal BglII site found at 86
    Illegal BamHI site found at 759
    Illegal XhoI site found at 90
    Illegal XhoI site found at 890
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 550
    Illegal XbaI site found at 83
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 550
    Illegal XbaI site found at 83
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

QB
Yao, Y., Zhang, W., Zhang, M., Jin, S., Guo, Y., Zu, Y., Ren, K., Wang, K., Chen, G., Lou, C., and Wu, Q. (2019) A Direct RNA-to-RNA Replication System for Enhanced Gene Expression in Bacteria. ACS Synth. Biol. 8, 1067–1078
Registry of Standard Biological Parts

References

QB
Refereces
[1]
Yao, Y., Zhang, W., Zhang, M., Jin, S., Guo, Y., Zu, Y., Ren, K., Wang, K., Chen, G., Lou, C., and Wu, Q. (2019) A Direct RNA-to-RNA Replication System for Enhanced Gene Expression in Bacteria. ACS Synth. Biol. 8, 1067–1078
Note, that the authors declared the following competing financial interest(s): Y. Y., W. Z., and Q. W. have filed a patent on RNA replication system and its usage.