Composite

Part:BBa_K3425060:Design

Designed by: Tereza Hubackova   Group: iGEM20_UofUppsala   (2020-10-25)


T7 polymerase regulated Juniper GFP substrate for Qβ replicase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 550
    Illegal XbaI site found at 83
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 550
    Illegal NheI site found at 859
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 550
    Illegal BglII site found at 86
    Illegal BamHI site found at 759
    Illegal XhoI site found at 90
    Illegal XhoI site found at 890
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 550
    Illegal XbaI site found at 83
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 550
    Illegal XbaI site found at 83
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

QB recognition sites
Yao, Y., Zhang, W., Zhang, M., Jin, S., Guo, Y., Zu, Y., Ren, K., Wang, K., Chen, G., Lou, C., and Wu, Q. (2019) A Direct RNA-to-RNA Replication System for Enhanced Gene Expression in Bacteria. ACS Synth. Biol. 8, 1067–1078
Registry of Standard Biological Parts

References

QB recognition sites
Refereces
[1]
Yao, Y., Zhang, W., Zhang, M., Jin, S., Guo, Y., Zu, Y., Ren, K., Wang, K., Chen, G., Lou, C., and Wu, Q. (2019) A Direct RNA-to-RNA Replication System for Enhanced Gene Expression in Bacteria. ACS Synth. Biol. 8, 1067–1078
Note, that the authors declared the following competing financial interest(s): Y. Y., W. Z., and Q. W. have filed a patent on RNA replication system and its usage.