Part:BBa_K3657034

RNA AUAGAU binding Pumby protein coupled with C-Terminal GFP

This is a fusion protein containing AUAGAU binding Pumby Protein (BBa_K3657004) and C-terminal Split-GFP (BBa_K3657028).

Usage and Biology

AUAGAU binding Pumby Protein binds specifically the AUAGAU RNA sequence(BBa_K3657015). Pumby Proteins can be designed to bind a specific RNA sequence.

C-terminal Split-GFP is a protein that combines to a fluorescent complex when in proximity of N-terminal Split-GFP (BBa_K3657027).

This construct was planned to be used to explore the ability to use RNA as a linker between proteins. Following linker should be used: BBa_K3657036. The part with the N-terminal of the Split-GFP planned for the experiment: BBa_K3657033. You can find out more about the planned experiment here: iGEM Heidelberg 2020.

Due to the COVID19 pandemics we did not obtain empirical data about this part.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 184
  Illegal XhoI site found at 224
  Illegal XhoI site found at 449
  Illegal XhoI site found at 634
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]