Composite

Part:BBa_K3425054

Designed by: Tereza Hubackova   Group: iGEM20_UofUppsala   (2020-10-25)
Revision as of 00:03, 28 October 2020 by Thomas A (Talk | contribs)


Constitutive expression construct of Juniper GFP

Asembled using Type IIS iGEM Loop assembly standardfrom the constitutive promoter, BBa_J23100, the RBS for Type IIS iGEM Standard Assembly, BBa_K3425031, the Juniper GFP for Type IIS iGEM standard assembly, BBa_K3425048, followed by the double terminator BBa_B0015.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 476
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 476
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 476
    Illegal BamHI site found at 685
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 476
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 476
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None