Part:BBa_K3411020
NarP Nitrate/Nitrite Sensor
Lambert_GA iGEM 2020's novel NarP system, BBa_K3411020, is also modeled after E.coli’s natural nitrite sensor in the NAR Operon. Similar to NarL, the promoter BBa_J23106, a constitutive Anderson Promoter, produces TetR, which represses the PtetO promoter, producing NarP. By regulating the amount of aTc, the molecule that inhibits TetR, the lab can control NarP’s expression, the product of the PtetO promoter. NarQ, the native membrane-bound protein, senses the amount of nitrate and/or nitrite in the cell: In the presence of nitrite and/or nitrate, NarQ will phosphorylate NarP, activating it, but if there is no nitrite and/or nitrate present, NarQ will not phosphorylate NarP. If NarP is phosphorylated, it will activate the nirB promoter, producing GFP.
Lambert_GA iGEM 2020's plans on cloning and characterizing our NarP composite part in the coming year as a part of our two-year project, AgroSense.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2000
Illegal NheI site found at 2023 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2727
Illegal AgeI site found at 1371 - 1000COMPATIBLE WITH RFC[1000]
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