Measurement

Part:BBa_K3475008

Designed by: LI SIYUAN   Group: iGEM20_Tongji_China   (2020-10-25)
Revision as of 22:41, 27 October 2020 by Night12138 (Talk | contribs)


Prm+ RBS GFP Ter

GFP activated by cI

Usage and Biology

T--Tongji China--202 4.jpg

FIGURE 4. The relative fluorescence of WT (E.coli-wildtype), J23110 (E.coli-pSB1C3-K608010), HrtO (E.coli-pUC-GW-HrtO-GFP) and I12007 (E.coli-pSB1C3-I12007-GFP-pSB1K3-HrtO-cI).
Measured by excitation wavelength of 475 nm, emission wavelength of 515 nm. Relative fluorescence is calculated by the formula below, LB medium as blank. Relative fluorescence is shown in logarithmic axis, 2 to the power of n.

T--Tongji China--202 t.jpg

FIGURE 5. Calculating formula.
As shown in the figure, the expression strength of I12007 is about 8% of HrtO, under the activation of cI (promoter: HrtO), showing 92% of loss during signal transformation from HBS to BAS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 738


[edit]
Categories
Parameters
None