Part:BBa_K3475008
Prm+ RBS GFP Ter
GFP activated by cI
Usage and Biology
FIGURE 6. The relative fluorescence of HrtR- (E.coli-pSB1K3-HrtO-cI-pA1c-R0051-mRFP-I12007-GFP), HrtR+ (E.coli-pHSB-cI-pA1c-R0051-mRFP-I12007-GFP) and WT (E.coli-wildtype).
Measured by excitation wavelength of 475 nm, emission wavelength of 515 nm for GFP; and by excitation wavelength of 588 nm, emission wavelength of 635 nm for mRFP. Relative fluorescence is calculated by the formula below, LB medium as blank.As shown in the figure, when heme is sufficient (simulated by KO of HrtR), the expression of GFP (promoter: I12007) is activated and the expression of RFP (promoter: R0051) is repressed; when heme lacks, the expression of GFP (promoter: I12007) is repressed and the expression of RFP (promoter: R0051) is activated. This can to some extent prove the engineering success of the connection between heme biosensor system and bilateral ARG system.
FIGURE 7. Calculating formula.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 738
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