Composite

Part:BBa_K3484003

Designed by: Eduard Sune & Andreu Pascuet   Group: iGEM20_UPF_Barcelona   (2020-10-26)
Revision as of 20:28, 27 October 2020 by Andreupf99 (Talk | contribs)


Intein-mediated T3(THRB) → eGFP (transcriptional unit)

This part is the transcriptional unit for BBa_K3484002. This part expresses constitutively an intein-mediated T3 biosensor with enhanced GFP (eGFP) as a reporter (IMT3_eGFP). This part was an intermediate sensor to later on develop the IMT3_sfGFP (BBa_K3484001). Visit its page to see the characterization and final design.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 235
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 725
    Illegal NgoMIV site found at 2228
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1640


[edit]
Categories
//chassis/prokaryote/ecoli
//function/reporter/fluorescence
Parameters
None