Composite
Part:BBa_K3484001
Designed by: Eduard Sune & Andreu Pascuet Group: iGEM20_UPF_Barcelona (2020-10-26)
Intein-mediated T3(THRB) → sfGFP (transcriptional unit)
This part is the transcriptional unit for BBa_K3484000. This part expresses constitutively an intein-mediated T3 biosensor. It is conformed by the Mycobacterium tuberculosis (Mtu) RecA intein, but replacing its endonuclease domain by the thyroid hormone receptor β (THR-β) ligand binding domain (LBD). The extein (the side proteins that the intein will splice and join) is conformed by a split super-folder GFP (sfGFP). In presence of T3, the intein domains splice the protein and full sfGFP is generated.
To see the full information and characterization, take a look at the BBa_K3484000 protein domain.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 235
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 722
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 278
Illegal SapI.rc site found at 1637
[edit]
Categories
Parameters
//chassis/prokaryote/ecoli
//function/reporter/fluorescence
//function/reporter/fluorescence
None |