Part:BBa_K3505012

AndersonJ23115:RBS GB compatible with A1-B2

Constitutive Anderson BBa_J23115with RBS BBa_B0034

AndersonJ23115 is a strong constitutive promoter that can be used to tune the expression level of constitutively expressed parts.

Design Notes

The sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 BBa_K3505007 and has overhangs compatible for Golden Braid cloning. This has position A1-B3.

Fig.2:The overhangs of this part in the Golden Braid Grammar

Fig.1:J23115 with RBS

Verification of cloning

Fig.1:((U=Uncut , C= Cut) Restriction digestion of AndersonJ23115 with : EcoRI (C1a & C2a),Expected bands : 2156 bp, XhoI (C1b & C2b),Expected bands : 1264 + 892 bp Positive result: The set of C1a and C1b (same sample)

Experimental Use and Experience

This part is used in BBa_K3505033 BBa_K3505035

Source

Synthesized by IDT.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 11
  Illegal NheI site found at 34
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]