Signalling

Part:BBa_K3411020

Designed by: Janet Standeven, Nikhita Lalwani, Sindhura Mettupalli, Kathy Ye, Sachintha Ashok, Saif Khan   Group: iGEM20_Lambert_GA   (2020-10-22)
Revision as of 01:18, 27 October 2020 by Msindhu01824 (Talk | contribs)


NarP Nitrate/Nitrite Sensor

Lambert_GA iGEM 2020's novel NarP system, BBa_K3411020, is also modeled after E.coli’s natural nitrite sensor in the NAR Operon. Similar to NarL, the promoter BBa_J23106, a constitutive Anderson Promoter, produces TetR, which represses the PtetO promoter, producing NarP. By regulating the amount of aTc, the molecule that inhibits TetR, our lab can control NarP’s expression, the product of the PtetO promoter. NarQ, the native membrane-bound protein, senses the amount of nitrate and/or nitrite in the cell: In the presence of nitrite and/or nitrate, NarQ will phosphorylate NarP, activating it, but if there is no nitrite and/or nitrate present, NarQ will not phosphorylate NarP. If NarP is phosphorylated, it will activate the nirB promoter, producing GFP.

Lambert_GA iGEM 2020's plans on cloning and characterizing our NarP composite part in the coming year as a part of our two-year project, AgroSense.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2000
    Illegal NheI site found at 2023
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2727
    Illegal AgeI site found at 1371
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None