Signalling

Part:BBa_K3411040

Designed by: Janet Standeven, Nikhita Lalwani, Sindhura Mettupalli, Kathy Ye, Sachintha Ashok, Saif Khan   Group: iGEM20_Lambert_GA   (2020-10-23)
Revision as of 00:50, 27 October 2020 by Msindhu01824 (Talk | contribs)


NarL Gene

Lambert_GA iGEM 2020's NarL system is modeled after E.coli’s natural nitrate sensor, the Nar Operon, which includes the NarL response regulator, and NarX, the membrane-bound sensor protein. The NarL/NarX system detects nitrate. This specific part is the isolated NarL gene.

The listed NarL’s role within Lambert_GA iGEM 2020’s (improved from HKUST-Rice 2015, BBa_K1682018) NarL System serves to accept the phosphate group from NarX. The promoter BBa_J23106, a constitutive Anderson Promoter, initiates the production of TetR. TetR represses the PtetO promoter, producing NarL. In the presence of nitrate, NarX phosphorylates NarL. Therefore, phosphorylated NarL measures nitrate levels.

Lambert_GA iGEM 2020 will continue cloning and begin characterization of our NarL part in the coming year as a part of our two-year project, AgroSense.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 174
    Illegal BglII site found at 342
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None