RNA

Part:BBa_K3657025

Designed by: Julia Langer   Group: iGEM20_Heidelberg   (2020-10-21)
Revision as of 00:08, 27 October 2020 by Liza ch (Talk | contribs)


RNA Part of an RNA·DNA-DNA triple helix + MS2-Stemloop

This part consists of a sequence that forms an RNA·DNA-DNA triple helix (DNA part: BBa_K3657023) and a sequence that forms an MS2 hairpin (BBa_K3113006).

Usage and Biology

The MS2 hairpin binds to the MS2 protein (BBa_K3218006). The triple helix forming part binds the DNA sequence specifically. In that way this part should theoretically attract a protein of interest fused to an MS2 protein to a specific region on DNA. Due to COVID19 pandemic we didn't manage to get any empirical evidence that this part functions as described above.
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Sequence and Features
Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

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Categories
Parameters
None