Generator

Part:BBa_K143082

Designed by: Chris Hirst   Group: iGEM08_Imperial_College   (2008-10-27)
Revision as of 21:56, 29 October 2008 by Jec105 (Talk | contribs)

Pveg-spoVG RFP expression construct


RFP gene under expresson of the Pveg promoter and spoVG RBS of B. subtilis, with a chloramphenicol resistance marker for ease of selection.


Also contains the 5' and 3' amyE integration sequences to allow integration into B. subtilis at the amyE locus.


This device was used by the Imperial iGEM 2008 team to characterise the Pveg promoter and spoVG RBS (Combined part BBa_K143053) as part of the Biofabricator subtilis project.


Sequence and Features



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2136
    Illegal AgeI site found at 2248
  • 1000
    COMPATIBLE WITH RFC[1000]


Part Characterisation

[edit]
Categories
//chassis/prokaryote/bsubtilis
Parameters
None