Generator
Pveg-spoVG

Part:BBa_K143053

Designed by: Chris Hirst   Group: iGEM08_Imperial_College   (2008-10-08)

Promoter Pveg and RBS spoVG for B. subtilis


Constitutive promoter veg(BBa_K143012) coupled to the strong Ribosome Binding Site spoVG(BBa_K143021) from B. subtilis.


Pveg-spoVG can be used in the context of a Ribosomes per second (RiPS) output generator


To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.


Sequence and Features



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Part Characterisation

Please see the testing constructs BBa_K143079 and BBa_K143082 for characterisation data.

References

<biblio>

  1. 1 https://parts.igem.org/Assembly:RBS-CDS_issues

</biblio>

[edit]
Categories
//chassis/prokaryote/bsubtilis
Parameters
None